Abstract
Regulation of the hypoxic response in humans is regulated by the post-translational hydroxylation of hypoxia inducible transcription factor; a recombinant form of a human prolyl-4-hydroxylase (PHD2) was characterised and shown to have an unexpectedly high affinity for, and to copurify with endogenous levels of, its Fe(ii) cofactor and 2-oxoglutarate cosubstrate.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Binding Sites
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Chromatography, Liquid
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Ferrous Compounds / chemistry*
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Humans
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Hypoxia-Inducible Factor 1 / chemistry*
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Ketoglutaric Acids / chemistry*
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Mass Spectrometry
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Models, Molecular
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Procollagen-Proline Dioxygenase / chemistry*
Substances
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Ferrous Compounds
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Hypoxia-Inducible Factor 1
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Ketoglutaric Acids
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Procollagen-Proline Dioxygenase