Abstract
Human B lymphocytes localize and differentiate within the microenvironment of lymphoid germinal centers. A frozen section binding assay was developed for the identification of those molecules involved in the adhesive interactions between B cells and lymphoid follicles. Activated human B cells and B cell lines were found to selectively adhere to germinal centers. The VLA-4 molecule on the lymphocyte and the adhesion molecule INCAM-110, expressed on follicular dendritic cells, supported this interaction. This cellular interaction model can be used for the study of how B cells differentiate.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antibodies, Monoclonal
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Antigens, CD / analysis
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B-Lymphocytes / cytology
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B-Lymphocytes / immunology*
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B-Lymphocytes / ultrastructure
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Cell Adhesion
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Cell Adhesion Molecules / immunology*
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Cells, Cultured
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Humans
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Palatine Tonsil / cytology
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Palatine Tonsil / immunology
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Receptors, Very Late Antigen / immunology*
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Spleen / immunology
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Vascular Cell Adhesion Molecule-1
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Cell Adhesion Molecules
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Receptors, Very Late Antigen
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Vascular Cell Adhesion Molecule-1