Abstract
Prokineticin 2, a cysteine-rich secreted protein, regulates diverse biological functions including the neurogenesis of olfactory bulb. Here we show that the PK2 gene is a functional target gene of proneural basic helix-loop-helix (bHLH) factors. Neurogenin 1 and MASH1 activate PK2 transcription by binding to E-box motifs on the PK2 promoter with the same set of E-boxes critical for another pair of bHLH factors, CLOCK and BMAL1, in the regulation of circadian clock. Our results establish PK2 as a common functional target gene for different bHLH transcriptional factors in mediating their respective functions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Basic Helix-Loop-Helix Transcription Factors / genetics
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Basic Helix-Loop-Helix Transcription Factors / physiology*
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Cell Differentiation
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Cell Movement
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Gastrointestinal Hormones / genetics*
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Gene Expression Regulation
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Mice
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Nerve Tissue Proteins / physiology
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Neuropeptides / genetics*
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Olfactory Bulb / physiology*
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Promoter Regions, Genetic
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Stem Cells / cytology
Substances
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Ascl1 protein, mouse
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Basic Helix-Loop-Helix Transcription Factors
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Gastrointestinal Hormones
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Nerve Tissue Proteins
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Neuropeptides
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Prok2 protein, mouse
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Neurog1 protein, mouse