Abstract
Tests based on bacteriophage replication enable rapid screening of Mycobacterium tuberculosis for drug resistance. We describe a novel broth-based colorimetric method for detecting phage replication. When clinical isolates were tested by this novel method, high concordance was observed with both the traditional phage assay and gene mutation analysis for detection of resistance to rifampin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibiotics, Antitubercular / pharmacology*
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Colorimetry
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DNA, Bacterial / genetics
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Drug Resistance, Bacterial / genetics
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Humans
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Microbial Sensitivity Tests / methods*
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Microbial Viability
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Mycobacteriophages / physiology
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Mycobacterium tuberculosis / drug effects*
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Mycobacterium tuberculosis / isolation & purification
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Mycobacterium tuberculosis / virology
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Rifampin / pharmacology*
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Statistics as Topic
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Tuberculosis / microbiology
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Viral Plaque Assay
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Virus Replication
Substances
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Antibiotics, Antitubercular
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DNA, Bacterial
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Rifampin