Abstract
On the basis of its inhibition by SB216763, we identified the multifunctional enzyme Glycogen Synthase Kinase 3beta (GSK3beta) as a central regulator for differentiation and cell survival of adult neural stem cells. Detected by proteomic approaches, members of the Wnt/beta-catenin signaling pathway appear to participate in enhanced neuronal differentiation and activated transcription of beta-catenin target genes during GSK3beta inhibition, associated with decreased apoptosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis
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Cell Differentiation*
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Cell Proliferation*
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Cerebral Ventricles / cytology*
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Electrophoresis, Gel, Two-Dimensional
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Glycogen Synthase Kinase 3 / physiology*
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Glycogen Synthase Kinase 3 beta
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Neurons / cytology*
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Neurons / enzymology
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Proteomics / methods
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Rats
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Stem Cells / cytology*
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Stem Cells / enzymology
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Wnt Proteins / metabolism
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beta Catenin / genetics
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beta Catenin / metabolism
Substances
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Wnt Proteins
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beta Catenin
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Glycogen Synthase Kinase 3 beta
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Gsk3b protein, rat
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Glycogen Synthase Kinase 3