Abstract
The RNase protection assay is a standard approach to determine mRNA levels of a gene of interest in different tissues, developmental stages, or times of the day. Splicing or promoter variants can be studied with specific probes. It is widely used in chronobiology to study the temporal profile of expression of circadian genes and the effects of genetic manipulation on these oscillations. Methods to generate the riboprobes and to perform the RNase protection assay itself are described in this chapter.
MeSH terms
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Alternative Splicing
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Antisense Elements (Genetics)
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Bacteriophage T7 / enzymology
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Bacteriophage T7 / genetics
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Circadian Rhythm / genetics
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DNA-Directed RNA Polymerases / genetics
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Molecular Probe Techniques
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Promoter Regions, Genetic
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RNA, Messenger / analysis*
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RNA, Messenger / genetics*
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RNA, Viral / analysis
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RNA, Viral / genetics
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Ribonucleases*
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Viral Proteins / genetics
Substances
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Antisense Elements (Genetics)
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RNA, Messenger
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RNA, Viral
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Viral Proteins
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bacteriophage T7 RNA polymerase
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DNA-Directed RNA Polymerases
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Ribonucleases