Abstract
The effects of fruiting body maturity on antioxidant activity and antioxidants production of the wild mushroom, Lactarius piperatus, were evaluated. Several biochemical assays were used to screen the antioxidant properties: reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, inhibition of erythrocytes hemolysis mediated by peroxyl radicals and inhibition of lipid peroxidation using the beta-carotene linoleate model system. The amounts of phenols, flavonoids, ascorbic acid, beta-carotene and lycopene present in the immature, mature and degraded fruiting bodies were also determined. The highest antioxidant contents and the lowest EC(50) values for antioxidant activity were obtained in the mature stage with immature spores.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antioxidants / analysis*
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Antioxidants / metabolism
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Antioxidants / pharmacology*
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Ascorbic Acid / analysis
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Ascorbic Acid / pharmacology
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Basidiomycota / chemistry*
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Biphenyl Compounds
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Carotenoids / analysis
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Carotenoids / pharmacology
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Dose-Response Relationship, Drug
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Erythrocytes / drug effects
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Flavonoids / analysis
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Flavonoids / pharmacology
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Food Analysis / methods*
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Free Radical Scavengers / metabolism
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Hydrazines
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Lipid Peroxidation / drug effects
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Lycopene
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Oxidation-Reduction
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Phenols / analysis
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Phenols / pharmacology
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Picrates
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Spores, Fungal / chemistry
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beta Carotene / analysis
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beta Carotene / pharmacology
Substances
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Antioxidants
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Biphenyl Compounds
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Flavonoids
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Free Radical Scavengers
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Hydrazines
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Phenols
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Picrates
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beta Carotene
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Carotenoids
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1,1-diphenyl-2-picrylhydrazyl
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Ascorbic Acid
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Lycopene