Integrated siphon-based metering and sedimentation of whole blood on a hydrophilic lab-on-a-disk

Biomed Microdevices. 2007 Oct;9(5):675-9. doi: 10.1007/s10544-007-9076-0.

Abstract

In this paper, we present a novel and fully integrated centrifugal microfluidic "lab-on-a-disk" for rapid colorimetric assays in human whole blood. All essential steps comprising blood sampling, metering, plasma extraction and the final optical detection are conducted within t=150 s in passive, globally hydrophilized structures which obviate the need for intricate local hydrophobic surface patterning. Our technology features a plasma extraction structure (V=500 nL, CV<5%) where the purified plasma (cRBC<0.11%) is centrifugally separated, metered by an overflow and subsequently extracted by a siphon-based principle through a hydrophilic extraction channel into the detection chamber.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Sedimentation
  • Blood*
  • Calibration
  • Centrifugation / methods*
  • Clinical Chemistry Tests / instrumentation*
  • Colorimetry / instrumentation
  • Colorimetry / methods
  • Equipment Design
  • Ethanol / blood
  • Humans
  • Microchemistry / instrumentation
  • Microchemistry / methods
  • Microfluidic Analytical Techniques / instrumentation*
  • Microfluidic Analytical Techniques / methods*

Substances

  • Ethanol