cDNA cloning of the HMGI-C phosphoprotein, a nuclear protein associated with neoplastic and undifferentiated phenotypes

G Manfioletti; V Giancotti; A Bandiera; E Buratti; P Sautière; P Cary; C Crane-Robinson; B Coles; G H Goodwin

doi:10.1093/nar/19.24.6793

cDNA cloning of the HMGI-C phosphoprotein, a nuclear protein associated with neoplastic and undifferentiated phenotypes

Nucleic Acids Res. 1991 Dec 25;19(24):6793-7. doi: 10.1093/nar/19.24.6793.

Authors

G Manfioletti¹, V Giancotti, A Bandiera, E Buratti, P Sautière, P Cary, C Crane-Robinson, B Coles, G H Goodwin

Affiliation

¹ Università di Trieste, Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Italy.

Abstract

The HMGI-C protein is a nuclear phosphoprotein expressed at high levels in transformed cells. The cDNA encoding the mouse protein has been isolated and the sequence of the encoded protein shows that it is related to the HMGY and I proteins, proteins which bind in the minor groove of DNA containing stretches of A and T. The HMGI-C protein has three short highly basic domains, an acidic C-terminal domain, and potential CDC2/p34 and casein kinase II phosphorylation sites. Analysis of mRNA levels demonstrate that the HMGI-C gene is not expressed in a variety of mouse tissues but is expressed in Lewis lung carcinoma cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Cell Differentiation / genetics
Chromatography, High Pressure Liquid
Cloning, Molecular
Gene Expression / genetics
HMGA2 Protein
High Mobility Group Proteins / chemistry
High Mobility Group Proteins / genetics*
Lung Neoplasms / genetics*
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Phosphoproteins / genetics*

Substances

HMGA2 Protein
High Mobility Group Proteins
Phosphoproteins

Associated data

GENBANK/S74918
GENBANK/S74919
GENBANK/S74920
GENBANK/S74921
GENBANK/S74922
GENBANK/S74923
GENBANK/S74924
GENBANK/S74925
GENBANK/S74926
GENBANK/X58380