Abstract
Plant isoamylase-type starch-debranching enzymes (ISAs) hydrolyze alpha-1,6-linkages in alpha-1,4/alpha-1,6-linked polyglucans. Two ISAs, designated PvISA1/2 and PvISA3, were purified from developing seeds of kidney bean by ammonium sulfate fractionation and several column chromatographic procedures. The enzymes displayed different substrate specificities for polyglucans: PvISA1/2 showed broad chain-length specificities, whereas PvISA3 liberated specific chains with a DP of 2 to 4.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Gene Expression Regulation, Enzymologic
-
Gene Expression Regulation, Plant
-
Isoamylase / genetics
-
Isoamylase / isolation & purification
-
Isoamylase / metabolism*
-
Isoenzymes / genetics
-
Isoenzymes / isolation & purification
-
Isoenzymes / metabolism
-
Phaseolus / enzymology*
-
Phaseolus / genetics
-
Seeds / enzymology
-
Seeds / genetics
-
Starch / metabolism*
-
Substrate Specificity
-
Transcription, Genetic / genetics
Substances
-
Isoenzymes
-
Starch
-
Isoamylase