Abstract
Transposon mutagenesis and plasmid complementation studies have identified two genes, fepD and fepG, which are essential for ferrienterobactin transport in Escherichia coli. These genes mapped in the enterobactin gene cluster and genetic evidence indicated that they are transcribed as part of an operon (fepD, fepG, fepC). The nucleotide sequence of fepD was determine; it could encode a hydrophobic 33.8 kDa protein with sequence homologies to other iron and vitamin B12 transport proteins. Also identified, between fepD and fepB, was an open reading frame (ORF43) with no detectable function; its 43 kDa protein product (P43) was seen on polyacrylamide gels. The fepD-C operon and ORF43 were divergently transcribed from a 110bp region containing a binding site for the repressor protein Fur.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacterial Outer Membrane Proteins / genetics*
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Base Sequence
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Binding Sites / genetics
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Carrier Proteins / genetics*
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Escherichia coli / genetics*
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Genetic Complementation Test
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Iron / metabolism
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Membrane Transport Proteins / genetics*
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Molecular Sequence Data
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Multigene Family / genetics
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Mutagenesis, Insertional
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Open Reading Frames / genetics
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Operon / genetics
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Plasmids / genetics
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Receptors, Cell Surface*
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Restriction Mapping
Substances
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Bacterial Outer Membrane Proteins
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Carrier Proteins
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Membrane Transport Proteins
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Receptors, Cell Surface
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enterobactin receptor
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Iron
Associated data
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GENBANK/D12728
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GENBANK/D12729
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GENBANK/D12730
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GENBANK/D12731
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GENBANK/D12732
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GENBANK/D12733
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GENBANK/S81541
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GENBANK/S83587
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GENBANK/X55648
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GENBANK/X59402