Abstract
The ability to distinguish and identify specific subcellular compartments is essential to understanding organelle function, biogenesis, and maintenance within cells and to defining protein trafficking pathways. Fluorescent dyes and/or fluorescently labeled lipid derivatives can be used to identify ER, Golgi complex, and mitochondria. Specific conditions for labeling each of these compartments are described.
MeSH terms
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4-Chloro-7-nitrobenzofurazan / analogs & derivatives
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4-Chloro-7-nitrobenzofurazan / analysis
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4-Chloro-7-nitrobenzofurazan / chemistry
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Animals
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Carbocyanines / analysis
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Carbocyanines / chemistry
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Cells, Cultured / ultrastructure
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Ceramides / analysis
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Ceramides / chemistry
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Coloring Agents / analysis
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Coloring Agents / chemistry
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Endoplasmic Reticulum / chemistry
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Endoplasmic Reticulum / ultrastructure*
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Fluorescent Dyes / analysis*
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Fluorescent Dyes / chemistry
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Golgi Apparatus / chemistry
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Golgi Apparatus / ultrastructure*
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Intracellular Membranes / chemistry
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Intracellular Membranes / ultrastructure
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Mitochondria / chemistry
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Mitochondria / ultrastructure*
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Rhodamines / analysis
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Rhodamines / chemistry
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Specimen Handling / methods
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Sphingosine / analogs & derivatives
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Sphingosine / analysis
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Sphingosine / chemistry
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Staining and Labeling / methods*
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Tissue Fixation / methods
Substances
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Carbocyanines
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Ceramides
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Coloring Agents
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Fluorescent Dyes
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N-(5-(5,7-dimethyl Bodipy)-1-pentanoyl)-erythrosphingosine
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Rhodamines
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3,3'-dihexyl-2,2'-oxacarbocyanine
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tetramethylrhodamine
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N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl sphingosine
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4-Chloro-7-nitrobenzofurazan
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Sphingosine