Abstract
The specification and maintenance of growth sites are tightly regulated during cell morphogenesis in all organisms. ROOT HAIR DEFECTIVE 2 reduced nicotinamide adenine dinucleotide phosphate (RHD2 NADPH) oxidase-derived reactive oxygen species (ROS) stimulate a Ca2+ influx into the cytoplasm that is required for root hair growth in Arabidopsis thaliana. We found that Ca2+, in turn, activated the RHD2 NADPH oxidase to produce ROS at the growing point in the root hair. Together, these components could establish a means of positive feedback regulation that maintains an active growth site in expanding root hair cells. Because the location and stability of growth sites predict the ultimate form of a plant cell, our findings demonstrate how a positive feedback mechanism involving RHD2, ROS, and Ca2+ can determine cell shape.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Substitution
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Arabidopsis / cytology
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Arabidopsis / growth & development
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Arabidopsis / metabolism*
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Arabidopsis Proteins / chemistry
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Calcium / metabolism*
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Cation Transport Proteins / metabolism
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Cell Line
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Cell Shape
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EF Hand Motifs
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Endocytosis
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Feedback, Physiological*
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Humans
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Marine Toxins
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Mutant Proteins / chemistry
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Mutant Proteins / metabolism
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NADPH Oxidases / chemistry
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NADPH Oxidases / genetics
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NADPH Oxidases / metabolism*
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Oxazoles / pharmacology
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Phosphorylation
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Plant Roots / cytology*
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Plant Roots / metabolism
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Protein Structure, Tertiary
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Reactive Oxygen Species / metabolism*
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Recombinant Fusion Proteins / metabolism
Substances
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Arabidopsis Proteins
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Cation Transport Proteins
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Marine Toxins
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Mutant Proteins
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Oxazoles
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Reactive Oxygen Species
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Recombinant Fusion Proteins
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SCN1 protein, Arabidopsis
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calyculin A
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Rhd2 protein, Arabidopsis
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NADPH Oxidases
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Calcium