Abstract
The measurement of single poly(ethylene glycol) (PEG) molecules interacting with individual bilayer lipid membrane-bound ion channels is presented. Measurements were performed within a polymer microfluidic system including an open-well bilayer lipid membrane formation site, integrated Ag/AgCl reference electrodes for on-chip electrical measurements, and multiple microchannels for independent ion channel and analyte delivery. Details of chip fabrication, bilayer membrane formation, and alpha-hemolysin ion channel incorporation are discussed, and measurements of interactions between the membrane-bound ion channels and single PEG molecules are presented.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacterial Toxins
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Biosensing Techniques / instrumentation
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Biosensing Techniques / methods*
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Electrochemistry
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Electrodes
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Hemolysin Proteins / analysis*
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Hemolysin Proteins / metabolism
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Ion Channels / chemistry*
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Lipid Bilayers / chemistry*
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Microfluidic Analytical Techniques / instrumentation
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Microfluidic Analytical Techniques / methods*
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Polyethylene Glycols / analysis*
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Polyethylene Glycols / chemistry
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Reproducibility of Results
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Sensitivity and Specificity
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Silver / chemistry
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Silver Compounds / chemistry
Substances
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Bacterial Toxins
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Hemolysin Proteins
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Ion Channels
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Lipid Bilayers
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Silver Compounds
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staphylococcal alpha-toxin
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Silver
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Polyethylene Glycols
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silver chloride