Abstract
The present study reports the antiinflammatory activity of a methanol extract isolated from the stem bark of Magnolia kobus (MK). MK potently inhibited lipopolysaccharide (LPS)-induced production of nitric oxide and interleukin-1beta (IL-1beta) in RAW 264.7 cells, a murine macrophage-like cell line. The secretion of tumor necrosis factor-alpha (TNF-alpha) was also suppressed in LPS-stimulated RAW 264.7 cells although the magnitude of inhibition was weaker than that of nitric oxide and IL-1beta. The mRNA expressions of inducible nitric oxide synthase (iNOS), IL-1beta and TNF-alpha were also suppressed by MK in LPS-stimulated RAW 264.7 cells. Further study demonstrated that LPS-induced DNA binding of AP-1 and phosphorylation of c-jun N-terminal kinase (JNK) were inhibited by MK treatment in RAW 264.7 cells, whereas phosphorylation of p38 mitogen-activated protein kinase was unaffected. Moreover, topical application of MK suppressed ear swelling in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin inflammation model. Collectively, these results suggest that MK exerts antiinflammatory effects in vitro and in vivo and this might be mediated, at least in part, by blocking AP-1 and JNK activation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Administration, Topical
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Animals
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Anti-Inflammatory Agents / administration & dosage
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Anti-Inflammatory Agents / isolation & purification*
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Anti-Inflammatory Agents / pharmacology
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Cell Line
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Cell Survival / drug effects
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DNA / chemistry
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DNA / metabolism
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Ear, External / drug effects
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Ear, External / pathology
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Edema / chemically induced
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Edema / pathology
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Gene Expression / drug effects
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Interleukin-1beta / metabolism
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JNK Mitogen-Activated Protein Kinases / metabolism
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Macrophages / metabolism
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Magnolia / chemistry*
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Methanol / chemistry
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Mice
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Mice, Inbred BALB C
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Nitric Oxide / metabolism
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Nitric Oxide Synthase Type II / genetics
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Nitric Oxide Synthase Type II / metabolism
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Phosphorylation
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Phytotherapy*
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Plant Bark / chemistry*
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Plant Extracts / administration & dosage
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Plant Extracts / isolation & purification*
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Plant Extracts / pharmacology
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Protein Binding / drug effects
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Transcription Factor AP-1 / genetics
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Transcription Factor AP-1 / metabolism
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Tumor Necrosis Factor-alpha / metabolism
Substances
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Anti-Inflammatory Agents
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Interleukin-1beta
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Lipopolysaccharides
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Plant Extracts
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Transcription Factor AP-1
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Tumor Necrosis Factor-alpha
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Nitric Oxide
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DNA
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Nitric Oxide Synthase Type II
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JNK Mitogen-Activated Protein Kinases
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Methanol