Polymerase chain reaction and transcription using locked nucleic acid nucleotide triphosphates

Rakesh N Veedu; Birte Vester; Jesper Wengel

doi:10.1021/ja801389n

Polymerase chain reaction and transcription using locked nucleic acid nucleotide triphosphates

J Am Chem Soc. 2008 Jul 2;130(26):8124-5. doi: 10.1021/ja801389n. Epub 2008 Jun 6.

Authors

Rakesh N Veedu¹, Birte Vester, Jesper Wengel

Affiliation

¹ Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, Odense M, 5230, Denmark.

PMID: 18533656
DOI: 10.1021/ja801389n

Abstract

Polymerase chain reaction amplification of a locked nucleic acid (LNA)-modified DNA strand and transcription reactions using LNA-A nucleoside 5'-triphosphate were successfully accomplished with DNA and RNA polymerases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenine Nucleotides
Base Sequence
DNA-Directed DNA Polymerase
DNA-Directed RNA Polymerases
Oligonucleotides / genetics*
Polymerase Chain Reaction*
Transcription, Genetic*

Substances

Adenine Nucleotides
Oligonucleotides
locked nucleic acid
DNA-Directed RNA Polymerases
DNA-Directed DNA Polymerase