Stimulation of osteoblasts with Emdogain increases the expression of specific mineralization markers

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 Aug;106(2):304-8. doi: 10.1016/j.tripleo.2008.02.033. Epub 2008 Jun 11.

Abstract

Objective: The purpose of this study was to determine the effects of enamel matrix derivative on mRNA expression of markers related to periodontal healing.

Study design: Murine osteoprogenitor cells (MC3T3-E1) were grown for 12 and 16 days in mineralization media and stimulated with 100 microg/mL Emdogain (EMD). Cell cultures treated with 2% and 10% fetal calf serum (FCS) served as control. The mRNA expression of bone sialoprotein (BSP), osteopontin (OPN), and runt-related protein 2 (Runx2) was analyzed by real-time polymerase chain reaction. One-way analysis of variance was used for statistical analysis.

Results: Stimulation with EMD significantly (P < .01) enhanced mRNA expression of BSP up to 13.9-fold and of OPN up to 3.2-fold at day 16 compared with the 2% FCS control. The expression of mRNA for transcription factor Runx2 was not significantly changed.

Conclusion: The beneficial effects seen in periodontal regeneration after treatment with EMD may be related to an increase of the mineralization markers BSP and OPN at mRNA level.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Calcification, Physiologic / drug effects*
  • Core Binding Factor Alpha 1 Subunit / analysis
  • Core Binding Factor Alpha 1 Subunit / drug effects
  • Dental Enamel Proteins / pharmacology*
  • Gene Expression Regulation / genetics
  • Integrin-Binding Sialoprotein
  • Mice
  • Osteoblasts / drug effects*
  • Osteopontin / analysis
  • Osteopontin / drug effects
  • RNA, Messenger / drug effects
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sialoglycoproteins / analysis
  • Sialoglycoproteins / drug effects

Substances

  • Core Binding Factor Alpha 1 Subunit
  • Dental Enamel Proteins
  • Ibsp protein, mouse
  • Integrin-Binding Sialoprotein
  • RNA, Messenger
  • Runx2 protein, mouse
  • Sialoglycoproteins
  • Spp1 protein, mouse
  • enamel matrix proteins
  • Osteopontin