Abstract
Five new steroids, namely griffinisterones F-I (1-4) and griffinipregnone (5) have been isolated from the octocoral Dendronephthya griffini. The structures of these compounds were elucidated by extensive spectroscopic analysis. Compounds 1-3 were found to significantly inhibit the accumulation of the pro-inflammatory iNOS protein of the LPS-stimulated RAW264.7 macrophage cells at 10 microM. At the same concentrations 2 also could significantly inhibit the accumulation of the pro-inflammatory COX-2 protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anthozoa / chemistry*
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Anti-Inflammatory Agents / chemistry
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Anti-Inflammatory Agents / isolation & purification*
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Anti-Inflammatory Agents / pharmacology
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Cells, Cultured
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Cyclooxygenase 2 / metabolism
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Glucocorticoids / chemistry
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Glucocorticoids / isolation & purification*
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Glucocorticoids / pharmacology
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Lipopolysaccharides / pharmacology
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Macrophages / drug effects
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Macrophages / metabolism
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Magnetic Resonance Spectroscopy
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Mice
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Molecular Structure
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Nitric Oxide Synthase Type II / metabolism
Substances
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Anti-Inflammatory Agents
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Glucocorticoids
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Lipopolysaccharides
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Nitric Oxide Synthase Type II
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Ptgs2 protein, mouse
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Cyclooxygenase 2