Anti-platelet effects of flavonoids and flavonoid-glycosides from Sophora japonica

Arch Pharm Res. 2008 Jul;31(7):886-90. doi: 10.1007/s12272-001-1242-1. Epub 2008 Aug 14.

Abstract

A methanol extract of Sophora japonica was subjected to anti-platelet activity guided fractionation affording the isolation of four flavonoids and six flavonoid-glycosides: biochanin A (1), irisolidone (2), genistein (3), sissotrin (4), sophorabioside (5), genistin (6), tectoridin (7), apigenin (8), quercitrin (9), and rutin (10). The structure of each compound was determined by a variety of spectroscopic methods. Among the compounds, 1, 3, and 7 showed approximately 2.5-6.5 fold greater inhibitory effects on arachidonic acid (AA) and U46619 induced platelet aggregation (IC50: 19.9 and 99.8 microM; 20.3 and 53.8 microM; 25.9 and 123.4 microM, respectively) than acetylsalicylic acid (ASA, IC50: 63.0 and 350.0 microM). Compound 2 was an approximately 22-40 fold stronger inhibitor than ASA on AA and U46619 induced aggregation (IC50: 1.6 and 15.6 microM, respectively).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / antagonists & inhibitors
  • 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / pharmacology
  • Adenosine Diphosphate / antagonists & inhibitors
  • Adenosine Diphosphate / pharmacology
  • Animals
  • Arachidonic Acid / antagonists & inhibitors
  • Arachidonic Acid / pharmacology
  • Collagen / antagonists & inhibitors
  • Collagen / pharmacology
  • Flavonoids / isolation & purification
  • Flavonoids / pharmacology*
  • Fruit / chemistry
  • Glycosides / isolation & purification
  • Glycosides / pharmacology
  • In Vitro Techniques
  • Magnetic Resonance Spectroscopy
  • Plant Leaves / chemistry
  • Plant Stems / chemistry
  • Platelet Aggregation / drug effects
  • Platelet Aggregation Inhibitors*
  • Rats
  • Rats, Sprague-Dawley
  • Sophora / chemistry*
  • Spectrophotometry, Infrared
  • Spectrophotometry, Ultraviolet
  • Thromboxane A2 / pharmacology

Substances

  • Flavonoids
  • Glycosides
  • Platelet Aggregation Inhibitors
  • Arachidonic Acid
  • Thromboxane A2
  • Adenosine Diphosphate
  • 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
  • Collagen