Abstract
A methanol extract of Sophora japonica was subjected to anti-platelet activity guided fractionation affording the isolation of four flavonoids and six flavonoid-glycosides: biochanin A (1), irisolidone (2), genistein (3), sissotrin (4), sophorabioside (5), genistin (6), tectoridin (7), apigenin (8), quercitrin (9), and rutin (10). The structure of each compound was determined by a variety of spectroscopic methods. Among the compounds, 1, 3, and 7 showed approximately 2.5-6.5 fold greater inhibitory effects on arachidonic acid (AA) and U46619 induced platelet aggregation (IC50: 19.9 and 99.8 microM; 20.3 and 53.8 microM; 25.9 and 123.4 microM, respectively) than acetylsalicylic acid (ASA, IC50: 63.0 and 350.0 microM). Compound 2 was an approximately 22-40 fold stronger inhibitor than ASA on AA and U46619 induced aggregation (IC50: 1.6 and 15.6 microM, respectively).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / antagonists & inhibitors
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15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / pharmacology
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Adenosine Diphosphate / antagonists & inhibitors
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Adenosine Diphosphate / pharmacology
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Animals
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Arachidonic Acid / antagonists & inhibitors
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Arachidonic Acid / pharmacology
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Collagen / antagonists & inhibitors
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Collagen / pharmacology
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Flavonoids / isolation & purification
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Flavonoids / pharmacology*
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Fruit / chemistry
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Glycosides / isolation & purification
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Glycosides / pharmacology
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In Vitro Techniques
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Magnetic Resonance Spectroscopy
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Plant Leaves / chemistry
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Plant Stems / chemistry
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Platelet Aggregation / drug effects
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Platelet Aggregation Inhibitors*
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Rats
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Rats, Sprague-Dawley
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Sophora / chemistry*
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Spectrophotometry, Infrared
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Spectrophotometry, Ultraviolet
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Thromboxane A2 / pharmacology
Substances
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Flavonoids
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Glycosides
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Platelet Aggregation Inhibitors
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Arachidonic Acid
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Thromboxane A2
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Adenosine Diphosphate
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15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
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Collagen