D-fructose detection based on the direct heterogeneous electron transfer reaction of fructose dehydrogenase adsorbed onto multi-walled carbon nanotubes synthesized on platinum electrode

Biosens Bioelectron. 2009 Jan 1;24(5):1184-8. doi: 10.1016/j.bios.2008.07.002. Epub 2008 Jul 12.

Abstract

Multi-walled carbon nanotubes (MWCNTs) were synthesized on platinum plate electrodes by the chemical vapor deposition (CVD) method. From the results of X-ray photoelectron spectroscopy and voltammetric investigation, the iron nanoparticles used as a catalyst for the MWCNT synthesis were enclosed with MWCNTs. The MWCNTs synthesized on the Pt plate (MWCNTs/Pt) electrode were immediately immersed into solutions of d-fructose dehydrogenase (FDH) to immobilize the enzyme onto the MWCNTs/Pt electrode surfaces. After the FDH was immobilized onto the MWCNTs/Pt electrode, a well-defined catalytic oxidation current based on FDH was observed from ca. -0.15V (versus Ag/AgCl/sat'd KCl), which was close to the redox potential of heme c as a prosthetic group of FDH. From an analysis of a plot of the catalytic current versus substrate, the calibration range for the fructose concentration was up to ca. 40mmoldm(-3), and the apparent Michaelis-Menten constant was evaluated to be 11+/-1mmoldm(-3).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods
  • Electrochemistry / instrumentation*
  • Electrochemistry / methods
  • Electron Transport
  • Enzymes, Immobilized / chemistry
  • Equipment Design
  • Equipment Failure Analysis
  • Fructose / analysis*
  • Fructose / chemistry
  • Microelectrodes*
  • Nanotechnology / instrumentation
  • Nanotechnology / methods
  • Nanotubes, Carbon / chemistry*
  • Nanotubes, Carbon / ultrastructure
  • Oxidoreductases / chemistry*
  • Platinum / chemistry*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Enzymes, Immobilized
  • Nanotubes, Carbon
  • Fructose
  • Platinum
  • Oxidoreductases