Determination of viable mammalian cells by luminol chemiluminescence using microperoxidase

Shiro Yamashoji

doi:10.1016/j.ab.2008.11.030

Determination of viable mammalian cells by luminol chemiluminescence using microperoxidase

Anal Biochem. 2009 Mar 1;386(1):119-20. doi: 10.1016/j.ab.2008.11.030. Epub 2008 Dec 3.

Author

Shiro Yamashoji¹

Affiliation

¹ Shizuoka Institute of Science and Technology, Fukuroi, Shizuoka, Japan. yamasho@ms.sist.ac.jp

PMID: 19103146
DOI: 10.1016/j.ab.2008.11.030

Abstract

Chemiluminescent assay for menadione-catalyzed H(2)O(2) production by mammalian cells was modified by luminol chemiluminescence with microperoxidase instead of peroxyoxalate chemiluminescence with carcinogenic fluorescent materials. Luminol can be used as a common chemiluminescent reagent for the determination of viable mammalian cells and bacteria.

Publication types

Evaluation Study

MeSH terms

Animals
Cell Count / methods*
Cell Survival*
Humans
Hydrogen Peroxide
Luminescent Measurements*
Luminol
Methods
Peroxidases*
Research Design
Vitamin K 3

Substances

Luminol
Vitamin K 3
Hydrogen Peroxide
Peroxidases
microperoxidase