Jasmonates act with salicylic acid to confer basal thermotolerance in Arabidopsis thaliana

Shannon M Clarke; Simona M Cristescu; Otto Miersch; Frans J M Harren; Claus Wasternack; Luis A J Mur

doi:10.1111/j.1469-8137.2008.02735.x

Jasmonates act with salicylic acid to confer basal thermotolerance in Arabidopsis thaliana

New Phytol. 2009;182(1):175-187. doi: 10.1111/j.1469-8137.2008.02735.x. Epub 2009 Jan 8.

Authors

Shannon M Clarke^{1

2}, Simona M Cristescu³, Otto Miersch⁴, Frans J M Harren³, Claus Wasternack⁴, Luis A J Mur⁵

Affiliations

¹ Biochemistry Department, University of Otago, PO Box 56, Dunedin, New Zealand.
² Present address: AgResearch Limited, Invermay Agricultural Centre, Puddle Alley, Private Bag 50034, Mosgiel, New Zealand.
³ Life Science Trace Gas Exchange Facility, Department of Molecular and Laser Physics, Radboud University, Heyendaalseweg 135, NL 6525 AJ Nijmegen. The Netherlands.
⁴ Leibniz-Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle/Saale, Germany.
⁵ Institute of Biological Sciences, University of Aberystwyth, SY23 3DA, UK.

PMID: 19140948
DOI: 10.1111/j.1469-8137.2008.02735.x

Abstract

* The cpr5-1 Arabidopsis thaliana mutant exhibits constitutive activation of salicylic acid (SA), jasmonic acid (JA) and ethylene (ET) signalling pathways and displays enhanced tolerance of heat stress (HS). * cpr5-1 crossed with jar1-1 (a JA-amino acid synthetase) was compromised in basal thermotolerance, as were the mutants opr3 (mutated in OPDA reductase3) and coi1-1 (affected in an E3 ubiquitin ligase F-box; a key JA-signalling component). In addition, heating wild-type Arabidopsis led to the accumulation of a range of jasmonates: JA, 12-oxophytodienoic acid (OPDA) and a JA-isoleucine (JA-Ile) conjugate. Exogenous application of methyl jasmonate protected wild-type Arabidopsis from HS. * Ethylene was rapidly produced during HS, with levels being modulated by both JA and SA. By contrast, the ethylene mutant ein2-1 conferred greater thermotolerance. * These data suggest that JA acts with SA, conferring basal thermotolerance while ET may act to promote cell death.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptation, Physiological / drug effects*
Arabidopsis / drug effects*
Arabidopsis / genetics
Arabidopsis / physiology*
Arabidopsis Proteins / metabolism
Cyclopentanes / metabolism
Cyclopentanes / pharmacology*
Ethylenes / biosynthesis
Fatty Acids, Unsaturated / metabolism
Gene Expression Regulation, Plant / drug effects
Heat-Shock Response / drug effects
Membrane Proteins / metabolism
Mutation / genetics
Nucleotidyltransferases / metabolism
Oxylipins / metabolism
Oxylipins / pharmacology*
Phenotype
RNA, Messenger / genetics
RNA, Messenger / metabolism
Salicylic Acid / pharmacology*
Signal Transduction / drug effects
Temperature*

Substances

Arabidopsis Proteins
COI1 protein, Arabidopsis
CPR5 protein, Arabidopsis
Cyclopentanes
Ethylenes
Fatty Acids, Unsaturated
Membrane Proteins
NPR1 protein, Arabidopsis
Oxylipins
RNA, Messenger
12-oxophytodienoic acid
jasmonic acid
ethylene
JAR1 protein, Arabidopsis
Nucleotidyltransferases
Salicylic Acid