Abstract
Small N-C terminally cyclic ribosomal peptides are common in nature, yet the mechanisms underlying their biosyntheses remain largely unknown. We recently identified candidate peptide cyclase genes in the metagenomes of marine animals. Here we report that PatG, a protease discovered in this analysis, cleaves variables, short peptides out of a precursor protein, and cyclizes these peptides in vivo and in vitro.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Biological Products / chemistry*
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Biological Products / metabolism
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Catalysis
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Cyanobacteria / enzymology
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Macrocyclic Compounds / chemistry*
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Macrocyclic Compounds / metabolism
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Peptide Hydrolases / chemistry*
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Peptide Hydrolases / metabolism
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Peptides, Cyclic / chemistry*
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Peptides, Cyclic / metabolism
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Protein Precursors / chemistry
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Protein Precursors / metabolism
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Ribosomal Proteins / chemistry*
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Ribosomal Proteins / metabolism
Substances
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Biological Products
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Macrocyclic Compounds
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Peptides, Cyclic
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Protein Precursors
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Ribosomal Proteins
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Peptide Hydrolases