Characterization of a microbial community capable of reducing perchlorate and nitrate in high salinity

Biotechnol Lett. 2009 Jul;31(7):959-66. doi: 10.1007/s10529-009-9960-1. Epub 2009 Mar 8.

Abstract

Denitrifying up-flow packed-bed bioreactors fed with perchlorate and nitrate allowed for the examination of the impact of a variety of salt conditions (up to 10% w/v NaCl) on the complete perchlorate and nitrate removal capacity of the reactor using activated sludge taken from a municipal wastewater treatment plant. Based on the evaluation of the microbial community in the bioreactor by cloning analysis, Clostridium sp. and a Rhodocyclaceae bacteria were identified as the dominant clones. This suggests that they may be tolerant to high salt and can reduce both nitrate and perchlorate in such conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / classification*
  • Bacteria / isolation & purification
  • Bacteria / metabolism*
  • Bioreactors / microbiology*
  • Clostridium / classification
  • Clostridium / isolation & purification
  • Clostridium / metabolism
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Nitrates / metabolism*
  • Perchlorates / metabolism*
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Rhodocyclaceae / classification
  • Rhodocyclaceae / isolation & purification
  • Rhodocyclaceae / metabolism
  • Salinity
  • Sequence Analysis, DNA
  • Sewage / microbiology

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Nitrates
  • Perchlorates
  • RNA, Ribosomal, 16S
  • Sewage
  • perchlorate