Outbreak of hepatitis E virus infection in Darfur, Sudan: effectiveness of real-time reverse transcription-PCR analysis of dried blood spots

J Clin Microbiol. 2009 Jun;47(6):1931-3. doi: 10.1128/JCM.02245-08. Epub 2009 Apr 1.

Abstract

Biological samples collected in refugee camps during an outbreak of hepatitis E were used to compare the accuracy of hepatitis E virus RNA amplification by real-time reverse transcription-PCR (RT-PCR) for sera and dried blood spots (concordance of 90.6%). Biological profiles (RT-PCR and serology) of asymptomatic individuals were also analyzed.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Blood / virology*
  • Child
  • Child, Preschool
  • Desiccation*
  • Disease Outbreaks*
  • Hepatitis Antibodies / blood
  • Hepatitis E / epidemiology*
  • Hepatitis E virus / isolation & purification*
  • Humans
  • Middle Aged
  • Refugees
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Serum / virology
  • Specimen Handling / methods*
  • Sudan / epidemiology
  • Young Adult

Substances

  • Hepatitis Antibodies