Abstract
To visualize native or non-engineered RNA in live cells with single-molecule sensitivity, we developed multiply labeled tetravalent RNA imaging probes (MTRIPs). When delivered with streptolysin O into living human epithelial cancer cells and primary chicken fibroblasts, MTRIPs allowed the accurate imaging of native mRNAs and a non-engineered viral RNA, of RNA co-localization with known RNA-binding proteins, and of RNA dynamics and interactions with stress granules.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actin-Related Protein 2 / genetics
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Actins / genetics
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Animals
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Avian Proteins / genetics
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Bacterial Proteins / pharmacology
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Biotin / chemistry
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Carbocyanines / chemistry
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Cell Line, Tumor
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Cell Membrane Permeability / drug effects
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Cell Survival
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Cells, Cultured
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Chick Embryo
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DNA-Binding Proteins / genetics
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Fibroblasts / metabolism
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Fluorescent Dyes / analysis
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Humans
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Image Processing, Computer-Assisted
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Microscopy, Confocal
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Microscopy, Fluorescence
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Molecular Probe Techniques*
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Oligonucleotide Probes / analysis
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Oligonucleotide Probes / chemistry
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Poly(A)-Binding Proteins / metabolism
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RNA / analysis*
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RNA / metabolism
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RNA, Messenger / analysis
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RNA, Messenger / metabolism
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RNA-Binding Proteins / genetics
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Respiratory Syncytial Virus, Human / genetics
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Streptavidin / chemistry
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Streptolysins / pharmacology
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T-Cell Intracellular Antigen-1
Substances
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Actin-Related Protein 2
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Actins
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Avian Proteins
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Bacterial Proteins
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Carbocyanines
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DNA-Binding Proteins
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Fluorescent Dyes
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Oligonucleotide Probes
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Poly(A)-Binding Proteins
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RNA, Messenger
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RNA-Binding Proteins
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Streptolysins
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T-Cell Intracellular Antigen-1
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TIA1 protein, human
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ZBP1 protein, Gallus gallus
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ZBP1 protein, human
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cyanine dye 3
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streptolysin O
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RNA
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Biotin
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Streptavidin