Circular dichroism for the analysis of protein-DNA interactions

P D Cary; G Geoff Kneale

doi:10.1007/978-1-60327-015-1_36

Circular dichroism for the analysis of protein-DNA interactions

Methods Mol Biol. 2009:543:613-24. doi: 10.1007/978-1-60327-015-1_36.

Authors

P D Cary¹, G Geoff Kneale

Affiliation

¹ Biophysics Laboratories, Institute of Biomedical and Biomolecular Science, St. Michael's Building, University of Portsmouth, Portsmouth PO1 2DT, UK.

PMID: 19378189
DOI: 10.1007/978-1-60327-015-1_36

Abstract

Circular dichroism (CD) is a well-established technique for the analysis of both protein and DNA structure. The analysis of protein-nucleic acid complexes presents greater challenges, but at wavelengths above 250 nm, the circular dichroism signal from the DNA predominates. Examples are given of the use of CD to examine structural changes to DNA induced by protein binding.

MeSH terms

Base Sequence
Circular Dichroism / methods*
DNA / metabolism*
Protein Binding
Proteins / metabolism*

Substances

Proteins
DNA