The advent of near-atomic resolution in single-particle electron microscopy

Annu Rev Biochem. 2009:78:723-42. doi: 10.1146/annurev.biochem.78.070507.140543.

Abstract

Single-particle electron microscopy (EM) can provide structural information for a large variety of biological molecules, ranging from small proteins to large macromolecular assemblies, without the need to produce crystals. The year 2008 has become a landmark year for single-particle EM as for the first time density maps have been produced at a resolution that made it possible to trace protein backbones or even to build atomic models. In this review, we highlight some of the recent successes achieved by single-particle EM and describe the individual steps involved in producing a density map by this technique. We also discuss some of the remaining challenges and areas, in which further advances would have a great impact on the results that can be achieved by single-particle EM.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Humans
  • Microscopy, Electron / instrumentation
  • Microscopy, Electron / methods*
  • Multiprotein Complexes / ultrastructure
  • Proteins / ultrastructure
  • Viruses / ultrastructure

Substances

  • Multiprotein Complexes
  • Proteins