Molecular characterization of Panton-Valentine leukocidin-positive community-acquired methicillin-resistant Staphylococcus aureus isolates in Egypt

Microbiol Res. 2010 Feb 28;165(2):152-62. doi: 10.1016/j.micres.2009.03.005. Epub 2009 Jul 17.

Abstract

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) expressing Panton-Valentine leukocidin (PVL) have been characterized worldwide, but it has not been done in Egypt. In this study, we analyzed the molecular characteristics of PVL+CA-MRSA in Egypt, compared their genetic patterns with that of PVL+ methicillin-susceptible S. aureus and PVL+CA-MRSA from different countries, and investigated the allelic variations among their lukS-PV and lukF-PV gene sequences. The prevalence of PVL+MRSA was 19.04%. They belonged to different genetic clones with multi-locus sequence types (MLST) 30, 80, and the novel type 1010. ST80 strains showed unique antibiotic resistance profile that was distinguishable from the European ST80 clone: they had no resistance to tetracycline and fusidic acid. Two nonsynonymous substitutions in the lukS-PV gene region and two haplotype variants among these isolates were detected. We revealed that PVL is a good marker for CA-MRSA infections and that PVL+ isolates belonged to different genotypes.

Publication types

  • Comparative Study

MeSH terms

  • Alleles
  • Amino Acid Substitution / genetics
  • Bacterial Proteins / genetics
  • Bacterial Toxins / biosynthesis*
  • Bacterial Typing Techniques
  • Cluster Analysis
  • Community-Acquired Infections / epidemiology*
  • Community-Acquired Infections / microbiology*
  • DNA Fingerprinting
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Egypt / epidemiology
  • Electrophoresis, Gel, Pulsed-Field
  • Exotoxins / biosynthesis*
  • Genotype
  • Humans
  • Leukocidins / biosynthesis*
  • Leukocidins / genetics
  • Methicillin-Resistant Staphylococcus aureus / classification*
  • Methicillin-Resistant Staphylococcus aureus / genetics*
  • Methicillin-Resistant Staphylococcus aureus / isolation & purification
  • Microbial Sensitivity Tests
  • Molecular Epidemiology
  • Molecular Sequence Data
  • Prevalence
  • Sequence Analysis, DNA
  • Staphylococcal Infections / epidemiology*
  • Staphylococcal Infections / microbiology*

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • DNA, Bacterial
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin
  • leukocidin S-component protein, Staphylococcus
  • LukF protein, Staphylococcus aureus

Associated data

  • GENBANK/FJ821790
  • GENBANK/FJ821791
  • GENBANK/FJ821792
  • GENBANK/FJ821793