An Asian dust event, also sometimes known as a Yellow Sand event, is a seasonal meteorological phenomenon affecting East Asia, typically in the early spring. Because of the significant ecological and health effects of these events on East Asia, and the large amount of dust that is transported from the desert in China to Korea and Japan, these events have been receiving increased attention. It is likely that these storms often provide long-range transport to various microorganisms. However, despite a certain level of attention to the chemical analysis of these storms, microbiological studies of Yellow Sand dust have been scarce. We collected a total of 30 microbiological air samples using a PM(2.5) cyclone sampler in Seoul, Korea from April 2007 to March 2008. Six of these samples were collected during Yellow Sand events, while 24 were from non-Yellow Sand events. Chemical analysis was performed on the samples using a thermal-optical transmittance (TOT) method. Total nucleic acids were also extracted, and the 16S rDNA was amplified by PCR and analyzed by denaturing gradient gel electrophoresis (DGGE). Dendrogram analysis, based on DGGE, indicated that the microbial profiles from the Yellow Sand were distinctive from those of the non-Yellow Sand samples. Microorganisms identified in Yellow Sand samples included Aquabacterium sp., Flavobacteriales bacterium sp., Prevotellaceae bacterium sp., and others, whereas microorganisms in non-Yellow Sand samples included Propionibacterium sp., Bacillus sp., Acinetobacter sp., and others. These results suggest that, as a result of Yellow Sand events, humans in the affected regions are exposed to communities of microorganisms that might cause various adverse health effects.