Abstract
An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Acetylene / chemistry
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Animals
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Azides / chemistry
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Carbonic Anhydrase II / antagonists & inhibitors
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Carbonic Anhydrase II / metabolism
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Carbonic Anhydrase Inhibitors / analysis
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Carbonic Anhydrase Inhibitors / pharmacology
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Cattle
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Microfluidic Analytical Techniques / instrumentation*
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Solid Phase Extraction
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Spectrometry, Mass, Electrospray Ionization
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Systems Integration*
Substances
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Azides
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Carbonic Anhydrase Inhibitors
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Carbonic Anhydrase II
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Acetylene