Development of a broadly reactive nested reverse transcription-PCR assay to detect murine noroviruses, and investigation of the prevalence of murine noroviruses in laboratory mice in Japan

Microbiol Immunol. 2009 Sep;53(9):531-4. doi: 10.1111/j.1348-0421.2009.00152.x.

Abstract

A broadly reactive nested RT-PCR assay to detect MNV was developed and subsequently used to investigate the prevalence of MNV in laboratory mice in Japan. MNV were detected in 8 (22%) of 37 murine stool specimens by second-round PCR, although no positive band was obtained from any specimen by first-round PCR. Genetic analysis of the second round PCR products showed that MNV sequences detected in this study were closely matched (97.2 approximately 99.1%) to that of MNV-3 (DQ223042). This is the first report demonstrating the prevalence of MNV in Japan.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Laboratory / virology
  • Caliciviridae Infections / epidemiology
  • Caliciviridae Infections / veterinary*
  • Caliciviridae Infections / virology
  • Japan / epidemiology
  • Mice
  • Molecular Sequence Data
  • Norovirus / classification
  • Norovirus / genetics
  • Norovirus / isolation & purification*
  • Phylogeny
  • Polymerase Chain Reaction / methods*
  • Reverse Transcription
  • Rodent Diseases / epidemiology
  • Rodent Diseases / virology*