Abstract
The open reading frame K'177, located at the left end of the African swine fever virus genome, codes for an early induced structural protein of 22,000 Da (p22), which is released from the viral particle by the nonionic detergent n-octyl-beta-D-glucopyranoside under conditions that solubilize external viral structural proteins. The predicted amino acid sequence of the protein contains a hydrophobic region at its N-terminus with characteristics of a signal peptide and, at early times after virus infection of Vero cells, the protein can be detected in the cell membrane by immunolabeling.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Western
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Classical Swine Fever Virus / genetics*
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Cloning, Molecular
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Escherichia coli / genetics
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Genes, Viral*
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Models, Molecular
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Molecular Sequence Data
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Open Reading Frames*
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Plasmids
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Protein Conformation
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Restriction Mapping
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Vero Cells
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Viral Structural Proteins / genetics*
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Viral Structural Proteins / isolation & purification
Substances
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Viral Structural Proteins
Associated data
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GENBANK/M57546
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GENBANK/M59045
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GENBANK/M59046
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GENBANK/M59047
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GENBANK/M59048
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GENBANK/M59049
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GENBANK/M59050
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GENBANK/M63908
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GENBANK/M63909
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GENBANK/M63910