Sulfation of tea polysaccharides: synthesis, characterization and hypoglycemic activity

Int J Biol Macromol. 2010 Mar 1;46(2):270-4. doi: 10.1016/j.ijbiomac.2009.12.007. Epub 2009 Dec 22.

Abstract

Neutral polysaccharides (NTPS) and acid polysaccharides (ATPS) from tea leaves were obtained on a D315 macroporous anion-exchange resin column chromatography. NTPS and ATPS were sulfated by the pyridine-sulfonic acid method to obtain NTPS-S and ATPS-S. It was found that NTPS was easier sulfated than ATPS. There are strong characteristic absorption peaks located in 1258 cm(-1), 1146 cm(-1), 832 cm(-1) and 617 cm(-1) in the FTIR spectra of sulfated polysaccharides. Sulfation of polysaccharides also affected the endothermic and exothermic peaks via the DSC scan analysis. The appearance of exothermic peaks in both NTPS-S and ATPS-S indicated that the redox reaction might happen. The comparative study of hypoglycemic effect on mice showed that the sulfation of polysaccharides significantly improved hypoglycemic activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Glucose / drug effects
  • Calorimetry, Differential Scanning
  • Carbohydrate Conformation
  • Hypoglycemia / drug therapy*
  • Male
  • Mice
  • Microscopy, Atomic Force
  • Polysaccharides / chemical synthesis*
  • Polysaccharides / chemistry
  • Polysaccharides / therapeutic use*
  • Spectrophotometry, Infrared
  • Sulfates / metabolism*
  • Tea / chemistry*

Substances

  • Blood Glucose
  • Polysaccharides
  • Sulfates
  • Tea