Extraction and characterization of beta-D-glucan from oat for industrial utilization

Int J Biol Macromol. 2010 Apr 1;46(3):304-9. doi: 10.1016/j.ijbiomac.2010.01.002. Epub 2010 Jan 18.

Abstract

Oat beta-D-glucan is a valuable functional ingredient having numerous industrial, nutritional and health benefits. Its extraction needs careful attention as extraction process may affect the physiochemical and functional properties of extracted beta-D-glucan. The present study aimed at analyzing the effect of extraction of beta-D-glucan gum pellets from oat cultivar followed by detailed chemical and functional analysis. Enzymatic extraction process resulted in highest yield and recovery. Chemical analysis revealed protein as a dominating impurity. The water binding capacity of the beta-D-glucan ranged between 3.14 and 4.52 g g(-1) of sample. beta-D-Glucan exhibited ideal foaming stability when appropriate extraction technique was used. The viscosity of beta-D-glucan gum ranged between 35.6 and 56.16 cp. The color analysis showed L* value of beta-D-glucan gum pellet ranged between 72.18 and 83.54. Phosphorus, potassium and calcium appeared as major minerals in beta-D-glucan gum whereas iron, manganese and copper appeared as minor minerals. FTIR spectroscopy also confirms the presence of beta-D-glucan, protein and other components in extracted beta-D-glucan gum pellets. Overall, extracted beta-D-glucan showed a good potential for industrial usage.

MeSH terms

  • Avena / chemistry*
  • Color
  • Flour / analysis
  • Industry / methods*
  • Minerals / analysis
  • Spectroscopy, Fourier Transform Infrared
  • Viscosity
  • Water
  • beta-Glucans / chemistry*
  • beta-Glucans / isolation & purification*

Substances

  • Minerals
  • beta-Glucans
  • Water
  • beta-glucan, (1-3)(1-4)-