Abstract
E-cadherin is a member of the cadherin family of Ca(2+)-dependent cell-cell adhesion molecules. p120-Catenin and delta-catenin are known to bind to similar juxtamembrane regions of E-cadherin, and p120-catenin is known to stabilize E-cadherin. However, the function of competition between p120-catenin and delta-catenin for E-cadherin has not been fully explained. In this report, we show that cells overexpressing delta-catenin contain less p120-catenin than control cells at the cell-cell interface and that this causes the relocalization of p120-catenin from the plasma membrane to the cytosol. We show that successful binding by either one to E-cadherin adversely affects the stability of the other.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / metabolism*
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Animals
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Binding, Competitive
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Cadherins / metabolism*
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Catenins / chemistry
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Catenins / genetics
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Catenins / metabolism*
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Cell Line, Tumor
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Cytosol / metabolism
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Delta Catenin
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Gene Expression Regulation, Neoplastic
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Humans
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Male
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Membrane Proteins / metabolism
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Mice
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NIH 3T3 Cells
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Neoplasm Proteins / metabolism*
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Prostatic Neoplasms / metabolism*
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Protein Binding
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Protein Isoforms / metabolism
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Protein Stability
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Protein Structure, Tertiary
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Protein Transport
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Recombinant Fusion Proteins / metabolism
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Up-Regulation
Substances
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Cadherins
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Catenins
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Membrane Proteins
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Neoplasm Proteins
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Protein Isoforms
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Recombinant Fusion Proteins
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Delta Catenin