Abstract
3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na(2)S(2)O(3) to a cell-free extract, catechol at 50 mM was carboxylated in the presence of 3 M KHCO(3) to 3,4-dihydroxybenzoic acid with a molar conversion ratio of 28% after 14 h at 30 degrees C.
MeSH terms
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4-Aminobenzoic Acid / metabolism
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Bacterial Proteins / metabolism*
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Bicarbonates / pharmacology
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Carboxy-Lyases / metabolism*
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Catechols / metabolism*
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Coenzymes / pharmacology
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Culture Media / chemistry
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Enterobacter cloacae / enzymology*
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Enterobacter cloacae / metabolism*
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Gallic Acid / metabolism
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Hydroxybenzoates / metabolism
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Potassium Compounds / pharmacology
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Stereoisomerism
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para-Aminobenzoates
Substances
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Bacterial Proteins
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Bicarbonates
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Catechols
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Coenzymes
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Culture Media
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Hydroxybenzoates
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Potassium Compounds
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para-Aminobenzoates
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Acedoben
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3-hydroxybenzoic acid
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protocatechuic acid
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Gallic Acid
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Carboxy-Lyases
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3,4-dihydroxybenzoate decarboxylase
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potassium bicarbonate
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catechol
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4-Aminobenzoic Acid