Evaluation of a modified microscopic direct diagnosis of dermatophytosis

J Microbiol Methods. 2010 May;81(2):205-7. doi: 10.1016/j.mimet.2010.03.003. Epub 2010 Mar 6.

Abstract

Here we present a modified protocol for dematophyte diagnosis, utilizing a simple centrifugation step to significantly decrease false-negative results of the original KOH direct microscopy-based technique. Although culture constitutes the gold-standard diagnosis, the time spent for results is a limit. Fast and low-cost techniques are important for infection screening in underdeveloped countries.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthrodermataceae / cytology
  • Arthrodermataceae / isolation & purification*
  • Centrifugation
  • Dermatomycoses / diagnosis*
  • False Negative Reactions
  • Humans
  • Microscopy / methods*
  • Mycology / methods*
  • Sensitivity and Specificity