Two types of carbon nanotube (CNT) based films were fabricated by adsorbing two different types of multiwalled carbon nanotubes (MWCNTs) on bacterial cellulose membrane templates. A bacterial cellulose membrane consists of ribbon-shaped nanofibrils that are arranged to form a porous 3D network. These characteristics lead to the uniform deposition of MWCNTs on the membrane structure and the final CNT based films with high surface areas. Two types of MWCNTs were used: (1) MWCNTs that had been purified by an acid treatment and (2) Ag-doped MWCNTs, which consisted of MWCNTs with Ag nanoparticles attached to their surfaces. The morphologies of the Ag-doped MWCNTs and CNT based films were examined using transmission electron microscopy. In addition, the electrical conductivity and electrochemical properties of the CNT based films were measured using a four-point probe and a cyclic voltammeter, respectively. The cyclic voltammetry data showed that the Ag particles attached to the MWCNT surfaces influenced the electrochemical properties of the CNT based films.