Abstract
The D-amino acid, D-aspartate, is abundant in the developing brain, yet its function is unknown. Addition of d-aspartate to hippocampal or cortical slices prepared from 8- to 9-day-old rats stimulated polyphosphoinositide (PI) hydrolysis to a slightly greater extent than L-glutamate. The action of D-aspartate was concentration-dependent with an apparent EC(50) value of 1 mM and a maximal stimulation of 6- and 20-fold in cortical and hippocampal slices, respectively. Stimulation of PI hydrolysis by D-aspartate was largely reduced by pharmacological blockade of mGlu5 metabotropic glutamate receptors with 2-methyl-6-(phenylethynyl)pyridine. These findings suggest that D-aspartate behaves as an endogenous agonist of mGlu5 receptors during early postnatal life.
Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Newborn
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Brain / drug effects
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Brain / growth & development
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Brain / metabolism
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Cerebral Cortex / drug effects
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Cerebral Cortex / growth & development
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Cerebral Cortex / metabolism*
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D-Aspartic Acid / metabolism*
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Excitatory Amino Acid Antagonists / pharmacology
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Glutamic Acid / metabolism
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Hippocampus / drug effects
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Hippocampus / growth & development
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Hippocampus / metabolism*
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Hydrolysis
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In Vitro Techniques
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Phosphatidylinositol Phosphates / metabolism*
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Pyridines / pharmacology
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Rats
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Rats, Sprague-Dawley
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Receptor, Metabotropic Glutamate 5
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Receptors, Metabotropic Glutamate / antagonists & inhibitors
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Receptors, Metabotropic Glutamate / metabolism*
Substances
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Excitatory Amino Acid Antagonists
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Phosphatidylinositol Phosphates
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Pyridines
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Receptor, Metabotropic Glutamate 5
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Receptors, Metabotropic Glutamate
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Glutamic Acid
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D-Aspartic Acid
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6-methyl-2-(phenylethynyl)pyridine