Abstract
We report the expression pattern and construction of a transgenic zebrafish line for a transcription factor involved in otic vesicle formation and skeletogenesis. The zinc finger transcription factor sp7 (formerly called osterix) is reported as a marker of osteoblasts. Using bacterial artificial chromosome (BAC)-mediated transgenesis, we generated a zebrafish transgenic line for studying skeletal development, Tg(sp7:EGFP)b1212. Using a zebrafish BAC, EGFP was introduced downstream of the regulatory regions of sp7 and injected into one cell-stage embryos. In this transgenic line, GFP expression reproduces endogenous sp7 gene expression in the otic placode and vesicle, and in forming skeletal structures. GFP-positive cells were also detected in adult fish, and were found associated with regenerating fin rays post-amputation. This line provides an essential tool for the further study of zebrafish otic vesicle formation and the development and regeneration of the skeleton.
(c) 2010 Wiley-Liss, Inc.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animal Fins / physiology
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Animal Fins / surgery
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Animals
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Animals, Genetically Modified
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Bone Regeneration / genetics*
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Chondrocytes / metabolism
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Chromosomes, Artificial, Bacterial / genetics
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Cloning, Molecular
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Ear / embryology
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Ear / growth & development
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Embryo, Nonmammalian / embryology
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Embryo, Nonmammalian / metabolism
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Gene Expression Regulation, Developmental
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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In Situ Hybridization
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Larva / genetics
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Larva / metabolism
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Organogenesis / genetics*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sp7 Transcription Factor
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Zebrafish / embryology
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Zebrafish / genetics*
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Zebrafish / growth & development
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Zebrafish Proteins / genetics*
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Zebrafish Proteins / metabolism
Substances
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RNA, Messenger
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Sp7 Transcription Factor
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Transcription Factors
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Zebrafish Proteins
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sp7 protein, zebrafish
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Green Fluorescent Proteins