A survey of quantitative real-time polymerase chain reaction internal reference genes for expression studies in Brassica napus

Xue Chen; Martin Truksa; Saleh Shah; Randall J Weselake

doi:10.1016/j.ab.2010.05.032

A survey of quantitative real-time polymerase chain reaction internal reference genes for expression studies in Brassica napus

Anal Biochem. 2010 Oct 1;405(1):138-40. doi: 10.1016/j.ab.2010.05.032. Epub 2010 Jun 1.

Authors

Xue Chen¹, Martin Truksa, Saleh Shah, Randall J Weselake

Affiliation

¹ Agricultural Lipid Biotechnology Program, Department of Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6H 2P5.

PMID: 20522329
DOI: 10.1016/j.ab.2010.05.032

Abstract

Eight reference genes of Brassica napus were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) data, focusing on vegetative tissues and developing embryos. Analyses of expression stability indicated that UP1, UBC9, UBC21, and TIP41 were the top four choices as stably expressed reference genes for vegetative tissues, whereas ACT7, UBC21, TIP41, and PP2A were the top four choices for maturing embryos. In addition, radiolabeling of overall messenger RNA (mRNA) of maturing embryos indicated that the expression patterns of the top four ranked reference genes reflected the overall mRNA content changes in maturing embryos.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brassica napus / genetics*
Embryonic Development / genetics
Gene Expression Profiling
Plant Proteins / genetics*
Plant Proteins / standards
Polymerase Chain Reaction / methods*
Polymerase Chain Reaction / standards
RNA, Messenger / metabolism
Reference Standards

Substances

Plant Proteins
RNA, Messenger