Abstract
Treatment of SK-N-SH cells with morphine and interleukin-1beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1β and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.
Copyright © 2010 Elsevier B.V. All rights reserved.
Publication types
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Comparative Study
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Research Support, N.I.H., Extramural
MeSH terms
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Cell Line, Tumor
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Down-Regulation / genetics
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Down-Regulation / immunology*
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Humans
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Interleukin-1beta / antagonists & inhibitors
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Interleukin-1beta / metabolism
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Interleukin-1beta / physiology*
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Morphine / metabolism
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Morphine / pharmacology*
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Neuroblastoma / immunology*
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Neuroblastoma / metabolism*
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Neuroblastoma / pathology
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RNA, Messenger / antagonists & inhibitors
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RNA, Messenger / biosynthesis
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Receptor Cross-Talk / drug effects
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Receptor Cross-Talk / immunology*
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Receptors, Interleukin-1 Type I / antagonists & inhibitors
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Receptors, Interleukin-1 Type I / physiology
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Receptors, Opioid, mu / agonists
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Receptors, Opioid, mu / antagonists & inhibitors
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Receptors, Opioid, mu / metabolism*
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Up-Regulation / genetics
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Up-Regulation / immunology*
Substances
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IL1R1 protein, human
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Interleukin-1beta
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RNA, Messenger
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Receptors, Interleukin-1 Type I
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Receptors, Opioid, mu
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Morphine