Dual regulation of mu opioid receptors in SK-N-SH neuroblastoma cells by morphine and interleukin-1β: evidence for opioid-immune crosstalk

J Neuroimmunol. 2010 Oct 8;227(1-2):26-34. doi: 10.1016/j.jneuroim.2010.06.007. Epub 2010 Jul 7.

Abstract

Treatment of SK-N-SH cells with morphine and interleukin-1beta (IL-1β) produced dual regulation of the mRNA for the human mu opioid receptor (MOR) protein. Morphine produced a decrease in the MOR mRNA while IL-1β increased it, as assessed by real-time quantitative PCR. These data were consistent with immunocytochemical studies of treated and untreated cells. Morphine-mediated down-regulation of MOR was blocked by naltrexone and IL-1β-induced up-regulation of MOR was blocked by interleukin-1 receptor type 1 antagonist. Immune-opioid crosstalk was examined by IL-1β and morphine co-treatment. These data are the first to show dual regulation of MOR in neuroblastoma cells.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Line, Tumor
  • Down-Regulation / genetics
  • Down-Regulation / immunology*
  • Humans
  • Interleukin-1beta / antagonists & inhibitors
  • Interleukin-1beta / metabolism
  • Interleukin-1beta / physiology*
  • Morphine / metabolism
  • Morphine / pharmacology*
  • Neuroblastoma / immunology*
  • Neuroblastoma / metabolism*
  • Neuroblastoma / pathology
  • RNA, Messenger / antagonists & inhibitors
  • RNA, Messenger / biosynthesis
  • Receptor Cross-Talk / drug effects
  • Receptor Cross-Talk / immunology*
  • Receptors, Interleukin-1 Type I / antagonists & inhibitors
  • Receptors, Interleukin-1 Type I / physiology
  • Receptors, Opioid, mu / agonists
  • Receptors, Opioid, mu / antagonists & inhibitors
  • Receptors, Opioid, mu / metabolism*
  • Up-Regulation / genetics
  • Up-Regulation / immunology*

Substances

  • IL1R1 protein, human
  • Interleukin-1beta
  • RNA, Messenger
  • Receptors, Interleukin-1 Type I
  • Receptors, Opioid, mu
  • Morphine