Facile synthesis of amino-silane modified superparamagnetic Fe3O4 nanoparticles and application for lipase immobilization

Yanjun Cui; Yanfeng Li; Yong Yang; Xiao Liu; Lin Lei; Lincheng Zhou; Fei Pan

doi:10.1016/j.jbiotec.2010.07.013

Facile synthesis of amino-silane modified superparamagnetic Fe3O4 nanoparticles and application for lipase immobilization

J Biotechnol. 2010 Oct 1;150(1):171-4. doi: 10.1016/j.jbiotec.2010.07.013. Epub 2010 Jul 16.

Authors

Yanjun Cui¹, Yanfeng Li, Yong Yang, Xiao Liu, Lin Lei, Lincheng Zhou, Fei Pan

Affiliation

¹ College of Chemistry and Chemical Engineering, State Key Laboratory of Applied Organic Chemistry, Institute of Biochemical Engineering & Environmental Technology, Lanzhou University, Lanzhou 730000, China.

PMID: 20638425
DOI: 10.1016/j.jbiotec.2010.07.013

Abstract

In present study, a facile prepared nano-sized magnetic support was successfully synthesized. Then this support was applied for lipase immobilization using glutaraldehyde as a coupling agent. Experimental data showed that the immobilized lipase exhibited good thermal stability and reusability. The lipase loading amount and activity recovery were found to be 43.6 mg/g support and 58.2%. Kinetic studies suggested it an acceptable degree of specificity retention for an immobilization process.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Enzyme Stability
Enzymes, Immobilized / chemistry
Enzymes, Immobilized / metabolism*
Equipment Reuse
Glutaral / chemistry
Kinetics
Lipase / chemistry
Lipase / metabolism*
Magnetite Nanoparticles / chemistry*
Olive Oil
Plant Oils / metabolism
Silanes / chemistry
Temperature

Substances

Enzymes, Immobilized
Magnetite Nanoparticles
Olive Oil
Plant Oils
Silanes
tetraethoxysilane
Lipase
Glutaral