Abstract
We have previously described a cis enhancing sequence (CES) in the gp41 region of the HIV-1 env gene. It could enhance HIV-1 Gag expression via both rev-dependent and CTE (constitutive transport element)-dependent pathways. We identified a functionally important and conserved region in the CES that contained a predicted binding sequence for an SR protein, ASF/SF2. We show here that ASF/SF2 bound to this sequence in an electrophoretic mobility shift assay and that the putative ASF/SF2-binding sequence was required for the enhancement of Gag expression by CES and might play a role in HIV-1 posttranscriptional regulation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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COS Cells
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Chlorocebus aethiops
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Conserved Sequence
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Gene Expression Regulation, Viral
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HIV-1 / genetics
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HIV-1 / metabolism*
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Humans
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Protein Binding
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RNA Processing, Post-Transcriptional
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RNA Splicing
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RNA, Viral
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RNA-Binding Proteins / chemistry
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism*
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Regulatory Elements, Transcriptional / genetics*
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Serine-Arginine Splicing Factors
Substances
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Nuclear Proteins
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RNA, Viral
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RNA-Binding Proteins
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Serine-Arginine Splicing Factors