Abstract
1. The blood clearance, organ distribution and tissue concentration of several native, homopolymerized, and IgG-conjugated 125I-labelled ribosome-inactivating proteins (RIPs) were determined in mice. 2. Native RIPs were cleared rapidly from blood, with half-lives of 4-8 min, and were concentrated mainly in the kidneys. 3. After conjugation to IgG the three RIPs studied showed increased blood half-lives and decreased concentrations in the kidneys. 4. The two homopolymers studied had blood half-lives and kidney concentrations intermediate to those of free and conjugated RIPs. 5. These results indicate that after IgG-conjugation the increased half-lives of the RIPs studied were at least in part due to the larger molecular size of the conjugates and to their lower renal excretion.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antimetabolites / metabolism
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Antimetabolites / pharmacokinetics
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Antineoplastic Agents, Phytogenic / blood
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Antineoplastic Agents, Phytogenic / metabolism
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Antineoplastic Agents, Phytogenic / pharmacokinetics
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Glycoproteins / blood
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Glycoproteins / metabolism
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Glycoproteins / pharmacokinetics
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Glycoproteins / physiology
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Immunoglobulin G / metabolism
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Immunotoxins / metabolism
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Immunotoxins / pharmacokinetics*
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Immunotoxins / pharmacology
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Kidney / metabolism
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Lung / metabolism
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Mice
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N-Glycosyl Hydrolases*
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Plant Proteins / blood
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Plant Proteins / metabolism
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Plant Proteins / pharmacokinetics
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Polymers / metabolism
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Polymers / pharmacology
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Protein Synthesis Inhibitors / blood
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Protein Synthesis Inhibitors / metabolism
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Protein Synthesis Inhibitors / pharmacokinetics*
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Ribosome Inactivating Proteins, Type 1
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Ribosome Inactivating Proteins, Type 2
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Saporins
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Tissue Distribution
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Toxins, Biological*
Substances
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Antimetabolites
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Antineoplastic Agents, Phytogenic
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Glycoproteins
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Immunoglobulin G
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Immunotoxins
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Plant Proteins
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Polymers
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Protein Synthesis Inhibitors
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Ribosome Inactivating Proteins, Type 1
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Ribosome Inactivating Proteins, Type 2
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Toxins, Biological
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bryodin
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trichokirin protein, Trichosanthes kirilowii
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GEL protein, Gelonium multiflorum
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N-Glycosyl Hydrolases
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Saporins
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pokeweed antiviral protein