Nine 10-(indol-3-yl)-[13]cytochalasans such as a new chaetoglobosin V (1); two new natural products, prochaetoglobosin III (2) and prochaetoglobosin III(ed) (3); six known chaetoglobosins B-D (4-6), F (7), and G (8) and isochaetoglobosin D (9) in addition to two known sterols, 24(R)-5alpha,8alpha-epidioxyergosta-6-22-diene-3beta-ol (10) and ergosterol (11), were isolated from the fungus Chaetomium elatum ChE01. The structures of these compounds were elucidated by spectroscopic methods. Compounds 1-9 showed cytotoxicity against the human breast cancer (IC(50) 2.54-21.29 microM) and cholangiocarcinoma cell lines (IC(50) 3.41-86.95 microM).