Diagnosis of prosthetic joint infection by beadmill processing of a periprosthetic specimen

Clin Microbiol Infect. 2011 Mar;17(3):447-50. doi: 10.1111/j.1469-0691.2010.03359.x. Epub 2010 Nov 5.

Abstract

We report a microbiological process for the documentation of prosthetic joint infection (PJI). Intraoperative periprosthetic tissue samples from 92 consecutive patients undergoing revision surgery for PJI were submitted to mechanized beadmill processing: specimens were aseptically collected in polypropylene vials, filled with sterile water and glass beads and submitted to mechanized agitation with a beadmill. The documentation rate of PJI following culture on solid and liquid media was 83.7% and the contamination rate 8.7%. Final documentation was obtained after overnight culture for 51.9% of cases and with 7 days of broth culture for all documented cases.

MeSH terms

  • Candidiasis / diagnosis*
  • Candidiasis / etiology
  • Cell Culture Techniques
  • Female
  • Gram-Negative Bacterial Infections / diagnosis*
  • Gram-Negative Bacterial Infections / etiology
  • Gram-Positive Bacterial Infections / diagnosis*
  • Gram-Positive Bacterial Infections / etiology
  • Humans
  • Joint Prosthesis / adverse effects*
  • Joint Prosthesis / microbiology
  • Male
  • Microbiological Techniques
  • Middle Aged
  • Prosthesis-Related Infections / diagnosis*
  • Prosthesis-Related Infections / etiology