In vitro BACE-1 inhibitory activity of resveratrol oligomers from the seed extract of Paeonia lactiflora

Planta Med. 2011 Mar;77(4):374-6. doi: 10.1055/s-0030-1250370. Epub 2010 Oct 1.

Abstract

A new resveratrol oligomer (1) together with eight related components (2- 9) were isolated from the seed extract of Paeonia lactiflora (Paeoniaceae) as active principles responsible for the inhibition of beta-site APP-cleaving enzyme 1 (BACE-1) in vitro. The chemical structure of 1 was established as (-)-7a,8a- CIS- ε-viniferin with the aid of spectroscopic analyses including NOESY experiments. All isolated resveratrol oligomers (1- 9) demonstrated significant inhibition on baculovirus-expressed BACE-1 in a dose-dependent manner, which was assessed by the FRET assay using Rh-EVNLDAEFK as a substrate in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyloid Precursor Protein Secretases / antagonists & inhibitors*
  • Aspartic Acid Endopeptidases / antagonists & inhibitors*
  • Baculoviridae
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / isolation & purification
  • Enzyme Inhibitors / pharmacology*
  • Molecular Structure
  • Paeonia / chemistry*
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Resveratrol
  • Seeds
  • Stilbenes / chemistry
  • Stilbenes / isolation & purification
  • Stilbenes / pharmacology*

Substances

  • 7a,8a-cis-epsilon-viniferin
  • Enzyme Inhibitors
  • Plant Extracts
  • Stilbenes
  • Amyloid Precursor Protein Secretases
  • Aspartic Acid Endopeptidases
  • BACE1 protein, human
  • Resveratrol