Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG)

James M Colborn; Michael Y Kosoy; Vladimir L Motin; Maxim V Telepnev; Gustavo Valbuena; Khin S Myint; Yuri Fofanov; Catherine Putonti; Chen Feng; Leonard Peruski

doi:10.1128/JCM.00470-10

Improved detection of Bartonella DNA in mammalian hosts and arthropod vectors by real-time PCR using the NADH dehydrogenase gamma subunit (nuoG)

J Clin Microbiol. 2010 Dec;48(12):4630-3. doi: 10.1128/JCM.00470-10. Epub 2010 Oct 6.

Authors

James M Colborn¹, Michael Y Kosoy, Vladimir L Motin, Maxim V Telepnev, Gustavo Valbuena, Khin S Myint, Yuri Fofanov, Catherine Putonti, Chen Feng, Leonard Peruski

Affiliation

¹ Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80521, USA.

Abstract

We used a whole-genome scanning technique to identify the NADH dehydrogenase gamma subunit (nuoG) primer set that is sensitive and specific enough to detect a diverse number of Bartonella species in a wide range of environmental samples yet maintains minimal cross-reactivity to mammalian host and arthropod vector organisms.

Publication types

Evaluation Study

MeSH terms

Animals
Arthropod Vectors / microbiology*
Bacteriological Techniques / methods*
Bartonella / genetics
Bartonella / isolation & purification*
Cluster Analysis
DNA Primers / genetics
DNA, Bacterial / genetics
Environmental Microbiology*
Genome, Bacterial
Humans
Mammals / microbiology*
NADH Dehydrogenase / genetics*
Phylogeny
Polymerase Chain Reaction / methods*
Protein Subunits / genetics
Sensitivity and Specificity
Sequence Analysis, DNA
Sequence Homology

Substances

DNA Primers
DNA, Bacterial
Protein Subunits
NADH Dehydrogenase